인제대학교의과대학 약물유전제연구센터

분자약물유전체 연구부

분자약물유전체 (Pharmaco genetics)

주요연구분야 약동학 관련 단백 유전자들의 유전적 다형성 연구
약동학 관련 단백 변이유전자의 기능 연구
약물반응 관련 단백의 발현조절 개인차 기전 연구
  - 약물반응 관련 단백의 발현조절 기전 연구
  - 유전자-환경 상호작용 연구
고속 변이유전자 검색기술 개발
SNP Discovery and Scoring
Targets: Phase I/II, Transporters, Receptors
Methods
  • Direct Sequencing
  • Scoring for functional SNPs
  • LD/Haplotype analysis
  • Ethnic comparison
Projects
  • CYP2C8
  • UGT2B7/15, SULT1A1/2
  • HNF1A, HIF1A, HNF6
HT Genotyping Techniques
Targets: Genes, Methods
Methods
  • Functional SNP/haplotype determination (SNP, htSNP, ethnics)
  • Selection of methods (Sequencing, PCR-RFLP, AS-PCR, Pyrosequencing, TaqMan, SNaPShot, Luminex, Chip, Illumina)
  • Simplex (SNP) vs. multiplex (haplotype, gene combination)
  • Validation and patenting
Projects
  • CYP1A2 SNaPShot
  • CYP2D6 SNaPShot/Luminex-based Chip
  • CYP2C9 TaqMan
  • OCTs Pyrosequencing, etc.
In Vitro Functional Genetics
Targets: Genes, Proteins, Drugs
Methods
  • Functional analysis of variant proteins
  • Functional analysis of transporters' functions (transwell, substrates scr)
  • Functional analysis of regulatory SNPs (promoter, splicing)
  • Baculovirus, oocyte, cell culture, in vitro translation, etc.
Projects
  • UGT1A4/7, CYP2D6, CYP2C8, etc.
  • Haloperidol metabolites, lamivudine
  • hOCTs, hOATs cell lines
Gene Regulations
Targets: Genes, Transcriptions
Methods
  • mRNA expression profiling
  • Protein expression profiling
  • Cell culture/chemicals
  • Protein degradations
Projects
  • mRNA and protein expression profiling in human livers
  • Functional variability of liver proteins (Phase I, Phase II)
  • Promoter studies
  • Drug interaction of induction (PXR-DDI, BCRP, salt)
Spliceome : New challenge for individual variations besides genetic variations
Targets: Genes, Proteins
Methods
  • Splicing variant identification
  • Putative ASV prediction
  • Individual variations of ASV expressions
  • Development of ASV expression quantification
Projects
  • ASV mRNA profiling in human livers
  • Association studies of ASV and phenotypes (PXR-CYPs)
  • CYP2C8, CYP2J2, etc

약물단백질체(Phamarcoproteomics)

주요연구분야 Phosphoproteomics
Interactomics
Phosphoproteomics
Interactomics

화학유전체학 (Chemical Genomics)