인제대학교의과대학 약물유전제연구센터

임상약물유전체 연구부

임상약물유전체학 (Clinical Pharmacogenomics)

주요연구분야 Tailored pharmacotherapy
Pharmacokinetic and pharmacodynamic study in relation to pharmacogenetics
Pharmacogenetic epidemiology
Pharmacogenetics in the drug discovery and development

약물분석/대사체학

주요연구분야 Drug Metabolism/Pharmacokinetics
PharmacoMetabolomics
Bioanalysis
DMPK Key Technology
항목 요소기술
In vitro metabolism Metabolic stability Metabolic stability in microsomes, hepatocytes, plasma, blood
Subcellular metabolism Hepatocytes, S9 fraction, cytosol, microsomes, intestine
Metabolic route and kinetics Reaction phenotyping and kinetics of Phase I (CYPs) and Phase II (UGT) enzymes
Interspecies comparative metabolism Monkey, dog, hamster, mouse, rabbit, rat
Metabolite Identification Metabolite structure ID, toxic metabolite ID
In vitro transport Permeability test Permeability in Caco-2, MDCK, LLC-PK1 cells
Cryopreserved hepatocytes uptake
Biliary excretion in sandwich cultured hepatocytes
Transport system and kinetics Uptake screening test in Xenopus oocytes
Efflux screening test in MDCK-MDR1, MRPs, BCRP
Pharmacogenetics study Transport activity in wild type and variant transporters
Drug-drug interaction Inhibition Screening of inhibitory potential of CYPs, UGTs, transporters
Estimation of IC50 (Ki) value
Mechanism-based inhibition IC50 shift assay, reversibility assay
Estimation of KI and kinact value
Induction Reporter assay
mRNA expression level in hepatocytes
Phenotyping assay using specific substrates
In vivo PK
(preclinical)
Mass balance Mass balance study using radioactive or non-radioactive compounds
Absorption Estimation of bioavailability
Distribution Tissue distribution (brain, liver, kidney etc)
Blood partitioning, Protein binding
Metabolic profiling Metabolites profiling and Met ID
Excretion Renal excretion, biliary excretion, enterohepatic circulation
In vivo PK
(human)
Translational Research In vitro to in vivo extrapolation (SimCYP)
Interspecies Pharmacokinetic Scaling
Consultation of decision making for further clinical development
Phase 0 study Microdosing study
Phase I study First in human study
Absolute bioavailability
Drug-drug interaction potential study (cocktail study)
Genotype based ADME study
Special population (Gender, nephropathy, hepatic failure, Elderly)
Bridging study