SNP Discovery and Scoring
- Targets: Phase I/II, Transporters, Receptors
- Methods
Direct Sequencing
Scoring for functional SNPs
LD/Haplotype analysis
Ethnic comparison
- Projects
CYP2C8
UGT2B7/15, SULT1A1/2
HNF1A, HIF1A, HNF6
HT Genotyping Techniques
- Targets: Genes, Methods
- Methods
Functional SNP/haplotype determination (SNP, htSNP, ethnics)
Selection of methods
(Sequencing, PCR-RFLP, AS-PCR, Pyrosequencing, TaqMan, SNaPShot, Luminex, Chip,
Illumina)
Simplex (SNP) vs. multiplex (haplotype, gene combination)
Validation and patenting
- Projects
CYP1A2 SNaPShot
CYP2D6 SNaPShot/Luminex-based Chip
CYP2C9 TaqMan
OCTs Pyrosequencing, etc.
In Vitro Functional Genetics
- Targets: Genes, Proteins, Drugs
- Methods
Functional analysis of variant proteins
Functional analysis of transporters' functions (transwell, substrates scr)
Functional analysis of regulatory SNPs (promoter, splicing)
Baculovirus, oocyte, cell culture, in vitro translation, etc.
- Projects
UGT1A4/7, CYP2D6, CYP2C8, etc.
Haloperidol metabolites, lamivudine
hOCTs, hOATs cell lines
Gene Regulations
- Targets: Genes, Transcriptions
- Methods
mRNA expression profiling
Protein expression profiling
Cell culture/chemicals
Protein degradations
- Projects
mRNA and protein expression profiling in human livers
Functional variability of liver proteins (Phase I, Phase II)
Promoter studies
Drug interaction of induction (PXR-DDI, BCRP, salt)
Spliceome : New challenge for individual variations besides genetic variations
- Targets: Genes, Proteins
- Method
Splicing variant identification
Putative ASV prediction
Individual variations of ASV expressions
Development of ASV expression quantification
- Project
ASV mRNA profiling in human livers
Association studies of ASV and phenotypes (PXR-CYPs)
CYP2C8, CYP2J2, etc |
