Genomics
Research
분자약물유전체 (Pharmaco genetics)
| 주요연구분야 | 약동학 관련 단백 유전자들의 유전적 다형성 연구 |
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| 약동학 관련 단백 변이유전자의 기능 연구 | |
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약물반응 관련 단백의 발현조절 개인차 기전 연구 - 약물반응 관련 단백의 발현조절 기전 연구 - 유전자-환경 상호작용 연구 |
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| 고속 변이유전자 검색기술 개발 |
- SNP Discovery and Scoring
- Targets: Phase I/II, Transporters, Receptors
- Methods
- Direct Sequencing
- Scoring for functional SNPs
- LD/Haplotype analysis
- Ethnic comparison
- Projects
- CYP2C8
- UGT2B7/15, SULT1A1/2
- HNF1A, HIF1A, HNF6
- HT Genotyping Techniques
- Targets: Genes, Methods
- Methods
- Functional SNP/haplotype determination (SNP, htSNP, ethnics)
- Selection of methods (Sequencing, PCR-RFLP, AS-PCR, Pyrosequencing, TaqMan, SNaPShot, Luminex, Chip, Illumina)
- Simplex (SNP) vs. multiplex (haplotype, gene combination)
- Validation and patenting
- Projects
- CYP1A2 SNaPShot
- CYP2D6 SNaPShot/Luminex-based Chip
- CYP2C9 TaqMan
- OCTs Pyrosequencing, etc.
- In Vitro Functional Genetics
- Targets: Genes, Proteins, Drugs
- Methods
- Functional analysis of variant proteins
- Functional analysis of transporters' functions (transwell, substrates scr)
- Functional analysis of regulatory SNPs (promoter, splicing)
- Baculovirus, oocyte, cell culture, in vitro translation, etc.
- Projects
- UGT1A4/7, CYP2D6, CYP2C8, etc.
- Haloperidol metabolites, lamivudine
- hOCTs, hOATs cell lines
- Gene Regulations
- Targets: Genes, Transcriptions
- Methods
- mRNA expression profiling
- Protein expression profiling
- Cell culture/chemicals
- Protein degradations
- Projects
- mRNA and protein expression profiling in human livers
- Functional variability of liver proteins (Phase I, Phase II)
- Promoter studies
- Drug interaction of induction (PXR-DDI, BCRP, salt)
- Spliceome : New challenge for individual variations besides genetic variations
- Targets: Genes, Proteins
- Methods
- Splicing variant identification
- Putative ASV prediction
- Individual variations of ASV expressions
- Development of ASV expression quantification
- Projects
- ASV mRNA profiling in human livers
- Association studies of ASV and phenotypes (PXR-CYPs)
- CYP2C8, CYP2J2, etc