| 지원과제 |
|
| 저자명 |
Gwak JS, Song T, Song JY, Yun YS, Choi IW, Jeong Y, Shin JG, Oh S |
| 학회정보 |
Biochem Biophys Res Commun (3):444-449 2.750 |
| 제 목 |
Isoreserpine promotes beta-catenin degradation via Siah-1 up-regulation in HCT116 colon cancer cells. |
| LINK |
www.ncbi.nlm.nih.gov/pubmed/19607803 |
| 게시일 |
2010-03-09 14:15:24 |
| 년도 |
2009-International |
| 내용 |
Aberrant accumulation of intracellular beta-catenin in intestinal epithelial cells is a frequent early event during the development of colon cancer. To identify small molecules that decrease the level of intracellular beta-catenin, we performed cell-based chemical screening using genetically engineered HEK293 reporter cells to detect compounds that inhibit TOPFlash reporter activity, which was stimulated by Wnt3a-conditioned medium. We found that isoreserpine promoted the degradation of intracellular beta-catenin by up-regulation of Siah-1 in HEK293 and HCT116 colon cancer cells. Moreover, isoreserpine repressed the expression of beta-catenin/T-cell factor (TCF)-dependent genes, such as cyclin D1 and c-myc, resulting in the suppression of HCT116 cell proliferation. Our findings suggest that isoreserpine can potentially be used as a chemotherapeutic agent against colon cancer. |
