Publications
Product
| 지원과제 | PRCDM |
| 저자명 | Gwak J, Cho M, Gong SJ, Won J, Kim DE, Kim EY, Lee SS, Kim M, Kim TK, Shin JG, Oh S. |
| 학회정보 | J Cell Sci. 2006 Nov 15;119(Pt 22):4702-9. |
| 제 목 | Protein-kinase-C-mediated {beta}-catenin phosphorylation negatively regulates the Wnt/{beta}-catenin pathway. |
| LINK | www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=.. |
| 게시일 | 2006-12-05 13:29:53 |
| 년도 | 2006-International |
| 내용 | Normally, the Wnt/beta-catenin pathway controls developmental processes and homeostasis, but abnormal activation of this pathway is a frequent event during the development of cancer. The key mechanism in regulation of the Wnt/beta-catenin pathway is the amino-terminal phosphorylation of beta-catenin, marking it for proteasomal degradation. Here we present small-molecule-based identification of protein kinase C (PKC)-mediated beta-catenin phosphorylation as a novel mechanism regulating the Wnt/beta-catenin pathway. We used a cell-based chemical screen to identify A23187, which inhibits the Wnt/beta-catenin pathway. PKC was activated by A23187 treatment and subsequently phosphorylated N-terminal serine (Ser) residues of beta-catenin, which promoted beta-catenin degradation. Moreover, the depletion of PKCalpha inhibited the phosphorylation and degradation of beta-catenin. Therefore, our findings suggest that the PKC pathway negatively regulates the beta-catenin level outside of the Wnt/beta-catenin pathway.
PMID: 17093267 |
