Publications
Product
| 지원과제 | |
| 저자명 | Park KH, Lee JR, Hahn HS, Kim YH, Bae CD, Yang JM, Oh S, Bae YJ, Kim DE, Hahn MJ. |
| 학회정보 | Chem Pharm Bull (Tokyo). 2006 Sep;54(9):1266-70. |
| 제 목 | Inhibitory effect of ammonium tetrathiotungstate on tyrosinase and its kinetic mechanism. |
| LINK | www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=.. |
| 게시일 | 2006-12-05 13:16:30 |
| 년도 | 2006-International |
| 내용 | Tyrosinase requires two copper ions at the active site, in order to oxidize phenols to catechols. In this study, the inhibitory effect of the copper-chelating compound, ammonium tetrathiotungstate (ATTT), on the tyrosinase activity was investigated. ATTT was determined to inactivate the activity of mushroom tyrosinase, in a dose-dependent manner. The kinetic substrate reaction revealed that ATTT functions as a kinetically competitive inhibitor in vitro, and that the enzyme-ATTT complex subsequently undergoes a reversible conformational change, resulting in the inactivation of tyrosinase. In human melanin-producing cells, ATTT evidenced a more profound tyrosinase-inhibitory effect than has been seen in the previously identified tyrosinase inhibitors, including kojic acid and hydroquinone. Our results may provide useful information for the development of whitening agent.
PMID: 16946532 |
